Picking & Printing

…if you need more than a hand.
 

HTS Colony Picking

 

  • Overview
  • Description
  • Service Range
  • Applications
  • Additional Notes
  • Quality Control
Picking of cDNA or Genomic Clones into Microplates
Organism specific picking systems
QTrays, plates, petri dishes or Omnitrays
Barcode labeled
Clone collections

 

Picking of cDNA or Genomic Clones into Microplates

PCC‘s HTS clone picking service enables the production of arrayed cDNA or genomic libraries in barcode-labeled 96-well or 384-well microplates. A defined number of clones is picked from a tray or dish. Subclones are cherry-picked into microplates using an integrated plate management system. PCC uses Genetix QPix2 XT colony picker systems, which can pick 3840 colonies per hour or at least 30,000 clones a day.

Picking of cDNA or Genomic Clones into Microplates

  • Transformations*
  • Plating of cell suspensions onto agar dishes
  • Selection: Post incubation, agar trays or dishes are placed on an illuminated area inside the instrument The trays are automatically imaged and the captured image analyzed to determine which colonies are to be picked´. Colonies are detected on the basis of size, shape, color (blue/white), roundness and proximity to neighbors.The images are then reviewed, parameters adjusted and colonies selected or de-selected on an individual basis
  • Picking of clones into barcode-labeled microplates
  • Library storage or delivery
  • * transformation can be done with DNA delivered by the customer or with PCC‘s DNA libraries.

    Picking of cDNA or Genomic Clones into Microplates

    Clone collections are a requirement for the generation of macroarrays or microarrays (DNA, protein and cell arrays). Additionally, clone collections are useful for the generation of thematic gene sets (tissue, disease, drug-effects, biological function, etc.) or for large scale PCR experiments, DNA or protein isolation of individual target clones.

    Picking of cDNA or Genomic Clones into Microplates

  • Picking of E. coli as well as yeast colonies possible
  • Picking from different types of plates e.g. petri dishes, omnitrays, Qtrays, well-plates
  • Data tracking possible
  • Picking of cDNA or Genomic Clones into Microplates

    PCC‘s colony picking service is fully automated and under conditions that reduce the risk of contaminations to a minimum. Additionally, all plates are checked by multiple quality control steps e.g. PCR confirmation and sequencing of randomly taken colonies from individual plates. All quality-control results are electronically documented and available to our customers on demand.


     

    High Density Arraying

     

    • Overview
    • Description
    • Service Range
    • Applications
    • Additional Notes
    • Quality Control
    Customized High Density Arrays
    50.000 proteins per membrane
    Target protein identification
    Up to 6 membranes per hour
    High density colony printing

     

    Customized High Density Arrays

    Customized macroarrays give access to high density colony/protein arrays on PVDF/nylon membranes. Colonies are automatically picked first into microplates and then arrayed with contact printing of solid pins onto nylon filters. Up to 50,000 clones can be arrayed onto a standard filter (22cm x22cm) compared to a few thousand when screening manually. Less than 1 hour for the printing of six filters.

    Customized High Density Arrays

  • Construction of protein expression cDNA library
  • cDNA library of interest is transformed into E. coli cells and plated onto selective media
  • Colonies are picked into microplates
  • After cultivation of the bacteria, colonies are arrayed onto PVDF membranes
  • Protein expression is induced on membranes
  • Protein release from colonies is initiated by cell gentle lysis
  • Membranes are washed, fixed, blocked and ready for use of antibody/protein/peptide/DNA interactions
  • Colonies are printed in replicates
  • Multiple controls are placed over the complete membrane on distinct positions
  • Customized High Density Arrays

  • Screening experiments, where radioactive, fluorescent or biotinylated probes are used to detect binding proteins.
  • Screening experiments with antibodies or complete sera to detect antigens.
  • Epitope mapping of antibodies
  • Screening of human serum/plasma for auto-antibodies
  • Protein identification, using antibodies/serum/plasma
  • Functional assays, e.g. phosphorylation, methylation, glycosylation
  • Identification of DNA/RNA-binding proteins.
  • Customized High Density Arrays

  • Printing of DNA, proteins, peptides and cells possible
  • Printing on different types of membranes (nylon, cellulose, glass etc.)
  • Customized High Density Arrays

    Randomly taken cDNA clones are confirmed by PCR and DNA sequencing. Macroarrays are quality controlled a) by tag specific antibodies for protein expression, b) by labelled control proteins for immobilisation c) by expressed control proteins for folding and activity. All procedures are completely documented and available on customer’s demand.


     
    Discuss your HT project...