Pathway Profiling Service

PROCOMCURE Biotech offers a broad selection of cell-based and biochemical screening services for the analysis of your compounds, as characterization of mode of action and functionality are crucial for the development of a viable drug. We established a flexible portfolio of methods, which can be customized to your need (see also cell viability and toxicity).

The cell-based pathway activity screening services enable a rapid, sensitive, and quantitative assessment of signal transduction pathway activation by measuring the effects on downstream transcription factors using a luciferase or a GFP reporter system. The inducible reporter constructs are under the control of specific transcriptional response elements, each of which is engineered to measure the increase and decrease of activity of one cellular pathway as a reaction to the addition of a compound.

 

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    Assay
    Choose your assay and get suggestions for complementary experiments
    Cell line
    Choose the appropriate cell line
    Setup
    Choose the concentration range of your compound and incubation time or ask our staff for suggestions
    Results
    The data is delivered in tables and graphs
    Compound
    Please provide us with data about solubility and concentration of your test compound

     

    Service Catalog

     

    Transcription FactorPathwayHow it works
    Androgen Receptor (AR)Androgen ReceptorThe androgen receptor (AR or NR3C4) as ligand-activated transcription factor is activated by androgenic hormones like testosterone.
    AP-1MAPK/JNKThe activator protein-1 (AP1) transcription factor is a hetero- or homo-dimeric complex that comprises members of the proto-oncogene Jun and Fos protein family and is responsible for many important processes like cell growth and proliferation, differentiation and apoptosis.
    C/EBPC/EBPThe C/EBP, CCAAT/enhancer-binding protein, is involved in the regulation of mitotic growth arrest and differentiation of many different cell types. Acts as repressor of E2F1.
    CREBcAMP/PKAThe cAMP response element (CRE) is a meeting point for many extracellular and intracellular signals, including cAMP, calcium, G-protein coupled receptors (GPCR) and neurotrophins.
    E2F/DP1Cell CycleThe E2F family of transcription factors is a key regulator of cell-cycle checkpoints and DNA replication in mammalian cells. The E2F protein is a major target of the retinoblastoma gene product (Rb) and the activity of E2F/pRb is connected with the progression from G1 to S phase of the cell cycle. It also mediates the p53-dependent apoptosis and C/EBP-dependent differentiation.
    Elk-1/SRFMAPK/ERKThe ternary complex factors, TCR and Elk-1, form a complex with the SRF over the serum response element (SRE), and activate expression of immediate early genes. The Elk-1 protein is phosphorylated by mitogen-activated protein kinase (MAPK), causing increased DNA binding, ternary complex formation, and transcriptional activation of target genes.
    Estrogen Receptor (ER)Estrogen ReceptorThe hormone and its receptor regulate many physiological processes including normal cell growth, and the development of the reproductive, central nervous and skeletal systems. Mutual transrepression occurs between the estrogen receptor and NFκB in a cell-type specific manner.
    Fast1TGFβThis transcriptional activator is required for the induction of the goosecoid promoter and initiated by TGFβ and activin signaling.
    Glucocorticoid Receptor (GR)Glucocorticoid ReceptorThe glucocorticoid receptor (GR) as a transcription factor binds to the glucocorticoid response element (GRE) and affects inflammatory, cardiovascular, metabolic, immunologic, and homeostatic functions.
    HNF1aGlucose MetabolismThe hepatocyte nuclear factor 1 alpha (HNF1a) modulates, amongst other functions, the expression of tissue specific genes in liver and pancreatic islet cells. It is also involved in glucose metabolism and diabetes.
    HOXA5DevelopmentThe homeobox protein HOXA5 is an important developmental transcription factor and has multiple functions in gene expression, differentiation, morphogenesis and tumorigenesis.
    Hypoxia-inducedible-factor-1 (HIF-1)HypoxiaThe hypoxia-inducible factor-1 (HIF-1) protein is a master transcriptional regulator of the adaptive response to hypoxia. It regulates the oxygen homeostasis and plays significant roles in cancer progression as well as in cardiovascular diseases.
    KFL1ErythropoiesisThe Kruppel-like factor 1 (KFL1 or EKFL) is a transcriptional regulator of the erythropoiesis.
    MybHematopoiesisMyb is an oncogen and plays an important role in the regulation of hematopoiesis.
    Myc/Maxc-MycThe c-Myc protein is a transcription factor that heterodimerizes with an obligatory partner, Max, and influences as a proto-oncogen many growth related genes.
    NFATPKC/Ca ++The NFAT family of transcription factors plays a pivotal role in the transcriptional regulation of cytokine genes and other genes critical for the immune response. Several pathways are associated with activation of the NFAT enhancer element, including calcineurin and protein kinase C.
    NFκBNFκBThe nuclear factor-kappaB (NFκB) is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli and therefore plays a key role in inflammation, immune response, cell proliferation and protection against apoptosis.
    Oct-1Oct-1The Oct-1 transcription factor has influence on the expression of some snRNAs and histones. It also enables the formation of a transcription complex for immediate early genes in case of a herpes simplex virus infection.
    p53DNA damagep53, as a famous tumor repressor, plays an important role in DNA repair, cell cycle arrest, and apoptosis.
    Pax3Pax3This transcription factor regulates cell proliferation, migration and apoptosis.
    Pax4Glucose MetabolismThe Pax4 transcription factor plays a vital role in the development and differentiation of pancreatic islet beta cells, hence has an effect on glucose metabolism. Pax4 and Pax6 bind to the same promoter site.
    RBP-JκNotchThe RBP-Jκ protein (CSL/CBF1/Su (H)/Lag1) is a transcription factor, and a direct downstream modulator of Notch signaling, a pathway involved in cell-cell communication.
    Retinoic Acid Receptor (RAR)Retinoic Acid ReceptorRetinoic Acid, a metabolite of Vitamin-A, affects gene transcription and modulates a wide variety of biological processes by binding its receptor (RAR), which is associated as a heterodimer to the retinoic acid response element (RARE).
    SMAD2/SMAD3/SMAD4TGFβTGFβ signaling induces phosphorylation and activation of the SMAD2 and SMAD3 proteins, which then form complexes with the mediator SMAD4. These SMAD complexes translocate to the nucleus, where they activate the expression of TGFβ-responsive genes, which are involved in many cellular processes, including cell cycle arrest, differentiation, homeostasis, and immunosuppression.
    Sox5ERKSox5 is involved in the embryonic development and cell fate determination. Among its related pathways is the ERK signaling pathway.
    STAT1/STAT1Interferon GammaThe Interferon gamma (IFN-γ) activation sequence (GAS) reporter measures the activation of STAT1 homodimer, a component of the JAK/STAT-signal transduction pathways, and IFN-γ signaling.
    STAT1/STAT2Type I InterferonType I interferons (IFN-a and -ß) mediate signaling through STAT1 and STAT2 components of the JAK/STAT-signal transduction pathways.
    TCF/LEFWntThe Wnt signaling pathway is very famous in cancer research and causes dephosphorylation, stabilization, and entry into the nucleus of β-catenin. The stabilized β-catenin complexes with the TCF/LEF transcription factors, leading to the activation of Wnt-responsive genes.
    TEAD1HippoThe Hippo signaling pathway, of which the TEAD1 plays a key role, is involved in tumor suppression by inducing apoptosis and preventing proliferation.
    XBP1ER stress responseThis transcription factor is important for the unfolded protein response, embryonic development, differentiation and many more.

     

     

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