Custom Antibody Service

A number of factors might come to your mind when you try to define a successful antibody project: affinity or specificity of an antibody to a certain antigen, performance of an antibody in a certain application (e.g., Western blot, immunofluorescence, immunohistochemistry, ELISA, immunoprecipitation, ChIP, flow cytometry), delivery time and cost. The relative importance of each consideration may vary from customer to customer, and from project to project. For this reason, we have designed a highly flexible and individualized program, which would deliver suitable antibodies for your specific experiment(s) and target protein(s). The key advantages of using our custom antibodies service are summarized below:

 

Variety of antibodies…

 

Monoclonal
Polyclonal
Modification Specific

 

… and animal hosts.

 

Mouse
Rat
Rabbit
Sheep
Camel

 

Monoclonal antibodies

Antigen TypeSourceCustomer ProvidesMultiple-Antigen OptionApplications
peptidessynthesissequenceavailable WB, ELISA, IF, IHC, ICC
soluble fragmentssynthesis or expressionsequence or cDNAavailableIP, ChIP, FC, functional
full-length proteins expressionsequence or cDNA* N/AChIP, FC, functional

Table 1. Available Antigen Options for Custom Monoclonal Antibody Service. Abbreviation for antibody applications: WB, Western blot; IF, immunofluorescence; IHC, immunohistochemistry; ICC, immunocytochemistry; IP, immunoprecipitation; ChIP, chromatin immunoprecipitation; FC, flow cytometry. *For full-length protein antigens, we suggest that customer supply cDNA for cloning and expression.

 

Polyclonal antibodies

Antigen TypeSourceCustomer ProvidesMultiple-Antigen OptionApplications
peptidessynthesissequenceavailableWB, ELISA, IF, IHC, ICC
soluble fragmentssynthesis or expressionsequence or cDNAavailableIP, ChIP, FC, functional**
full-length proteinsexpressionsequence or cDNA*N/AChIP, FC, functional**

Table 2. Available Antigen Options for Custom Polyclonal Antibody Service. Abbreviation for antibody applications: WB, Western blot; IF, immunofluorescence; IHC, immunohistochemistry; ICC, immunocytochemistry; IP, immunoprecipitation; ChIP, chromatin immunoprecipitation; FC, flow cytometry. *For full-length protein antigens, we suggest that customer supply cDNA for cloning and expression. **We would usually recommend an AccuMono monoclonal package for IP, ChIP, FC and functional assays.

 

Discuss your custom antibody needs...